The San Francisco 49ers of the National Football League (NFL) are the longest-tenured major professional sports franchise in the city. The team began play in 1946 as an All-America Football Conference (AAFC) league charter member, moved to the NFL in 1950 and into Candlestick Park in 1971. The 49ers won five Super Bowl titles in the 1980s and 1990s behind coaches Bill Walsh and George Seifert & stars Joe Montana, Steve Young, Ronnie Lott, and Jerry Rice.

Major League Baseball's San Francisco Giants left New York for California prior to the 1958 season. Though boasting stars such as Willie Mays, Willie McCovey and Barry Bonds, the club did not win a World Series while based in San Francisco until 2010. The Oakland Athletics swept the Giants in the 1989 World Series, after Game 3 in San Francisco was infamously pre-empted by the Loma Prieta earthquake. The Giants play at AT&T Park, which opened in 2000, a cornerstone project of the South Beach and Mission Bay redevelopment.

At the collegiate level, the Dons of the University of San Francisco compete in NCAA Division I, where Bill Russell guided the program to basketball championships in 1955 and 1956. The San Francisco State Gators and the Academy of Art University Urban Knights compete in Division II. AT&T Park has since 2002 hosted an annual post-season college football bowl game, currently named the Kraft Fight Hunger Bowl.

The Bay to Breakers footrace, held annually since 1912, is best known for colorful costumes and a celebratory community spirit. The San Francisco Marathon attracts more than 21,000 participants. The Escape from Alcatraz triathlon has, since 1980, attracted 2,000 top professional and amateur triathletes for its annual race. The Olympic Club, founded in 1860, is the oldest athletic club in the United States. Its private golf course, situated on the border with Daly City, has hosted the U.S. Open on four occasions. The public Harding Park Golf Course is an occasional stop on the PGA Tour.

With an ideal climate for outdoor activities, San Francisco has ample resources and opportunities for amateur and participatory sports and recreation. There are more than 200 miles (320 km) of bicycle paths, lanes and bike routes in the city, and the Embarcadero and Marina Green are favored sites for skateboarding. Extensive public tennis facilities are available in Golden Gate Park and Dolores Park, as well as at smaller neighborhood courts throughout the city. Boating, sailing, windsurfing and kitesurfing are among the popular activities on San Francisco Bay, and the city maintains a yacht harbor in the Marina District. San Francisco residents have often ranked among the fittest in the U.S.